KMID : 0371320080740050323
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Journal of the Korean Surgical Society 2008 Volume.74 No. 5 p.323 ~ p.329
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Low Dose Carbon Monoxide Inhalation Prevents Chronic Allograft Nephropathy following Kidney Transplantation in Rats. Heme Oxygenase-1 Derivatives Study I
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Yoon Myung-Hee
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Abstract
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Purpose: Carbon monoxide (CO), a product of heme catalysis by heme oxygenase (HO-1, HO-2, HO-3), induces cytoprotection against ischemia/reperfusion (I/R) injury in a variety of organs such as the heart, lungs, kidneys, and liver. I examined whether CO would prevent chronic allograft nephropathy (CAN) associated with renal transplantation in rats.
Methods: Kidneys from male Fisher rats were perfused and harvested for transplantation. Lewis rats were used as recipients. After reperfusion of the implanted kidney, the recipient¡¯s remaining kidney was removed promptly. Recipients were then immediately treated with the indicated regimen of CO in a Plexiglas exposure chamber. At 90 days after transplantation, the animals were sacrificed for graft histopathology, serum creatinine, and blood urea nitrogen (BUN) as markers of kidney function.
Results: CAN in rats was achieved using a model of Fisher-to-Lewis transplants and evaluating kidney function over the 90 days following transplantation. CO administered at 100 ppm for 1 hr/day for 7 days prevented CAN at 90 days post-transplant. CO also decreased histopathological alterations, including leukocyte infiltration and cell death.
Conclusion: These data expand our understanding of the protective effects of low-dose CO inhalation in preventing the development of chronic fibro-inflammatory changes associated with chronic allograft nephropathy and allow us to devise methods for improving long-term renal allograft function, (J Korean Surg Soc 2008;74:323-329)
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KEYWORD
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Chronic allograft nephropathy, Low dose carbon monoxide, Kidney transplantation
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